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glass micropipette  (World Precision Instruments)


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    Structured Review

    World Precision Instruments glass micropipette
    Glass Micropipette, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 225 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glass+micropipette/Nanoliter2020+Injector+for+Nanoliter+Precision/pmc13674656-68-6-15
    Average 96 stars, based on 225 article reviews
    glass micropipette - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Striatal cholinergic interneuron pause response requires Kv1 channels, is absent in dyskinetic mice, and is restored by dopamine D5 receptor inverse agonism
    Article Snippet: A CaMKII-driven ChR2-EYFP expressing adeno-associated virus (rAAV2/CaMKII-hChR2(H134R)-EYFP-WPRE-PA) was injected in the thalamus at the following coordinates: –1.3 mm posterior from Bregma, 0.8 mm lateral, and –3 mm ventral from dura ( ). .. 0.5 μl of viral vector was delivered using a glass micropipette (World Precision Instruments) pulled with a vertical glass puller (Narishige). ..

    Article Title: Pannexin-1 channel activity regulates neurogenesis and cell survival in the developing cortex
    Article Snippet: Kits were placed on a stereotaxic frame (Stoelting, Cat. #51615) on top of a warming pad (Stoelting, Cat. #51625W) to maintain internal temperature. .. A plasmid mixture of either WT or mutant transposon (2.5μg/μl), pCAG-PBase (500ng/μl), pCAG-dsRed (1μg/μl), and 0.1% FastGreen FCF (Sigma, Cat. F7252-5G) was injected (∼3 ul) through a pulled glass micropipette (World Precision Instruments, Cat. #1B120F-4) at 2 mm below pia into the lateral (right) telencephalic ventricle. .. Following intraventricular injection, tweezers with needle and paddle electrodes (NEPA Gene, Cat. #CUY661-3X7) were used to electroporate the DNA into the ventricular zone of the developing cortex by delivering five 50-ms electric pulses of 50 V at 1-s intervals using an electroporator (BTX, Cat. #ECM 830).

    Glass:

    Article Title: Striatal cholinergic interneuron pause response requires Kv1 channels, is absent in dyskinetic mice, and is restored by dopamine D5 receptor inverse agonism
    Article Snippet: A CaMKII-driven ChR2-EYFP expressing adeno-associated virus (rAAV2/CaMKII-hChR2(H134R)-EYFP-WPRE-PA) was injected in the thalamus at the following coordinates: –1.3 mm posterior from Bregma, 0.8 mm lateral, and –3 mm ventral from dura ( ). .. 0.5 μl of viral vector was delivered using a glass micropipette (World Precision Instruments) pulled with a vertical glass puller (Narishige). ..

    Article Title: In vivo reprogramming of NG2 glia improves bladder function after spinal cord injury
    Article Snippet: 33 gauge, 18-degree-beveled needle , Hamilton, Reno, NV , Cat# 22033 (Customized). .. Glass micropipette , World Precision Instruments , Cat# 504949. .. Nanoliter 2020 Injector , World Precision Instruments , Cat# NANOLITER2020.

    Article Title: Systematic evaluation of AAV administration strategies for targeting Phox2b + primary visceral sensory neurons
    Article Snippet: .. Injections were performed using a pulled glass micropipette connected to a Nanoliter 2020 injector (300704, World Precision Instruments). ..

    Bioprocessing:

    Article Title: Functional role of dentate gyrus calbindin-D28K in supporting neurogenesis and emotional-social behavior relevant to antidepressant action.
    Article Snippet: Under anesthesia with a mixture of medetomidine (0.3 mg/ kg, ZENOAQ, Japan, #28-0102), midazolam (4 mg/kg, Sandoz, Japan, #614243022), and butorphanol (5 mg/kg, Meiji, Japan), mouse brains were fixed using stereotaxic instruments (Narishige, Tokyo, Japan, SR-5 M-HT). .. An AAV solution of 500 nL per injection site (2.5 × 108 copies) was bilaterally injected into the DG using a Pneumatic PicoPump (World Precision Instruments, Hessen, Germany, PV820) through a glass micropipette (World Precision Instruments, 1B100–3) with a micropipette puller (Narishige, PN-30). ..

    Article Title: Combination of engineered cell type-specific promoters and a high-efficiency AAV capsid restores hearing in adult DFNB1 mice model with demonstrated safety in nonhuman primate
    Article Snippet: .. A total of 2 μL of AAV-ie-EGFP, 2 μL of AAV-ie-mNeonGreen-Bpnls or 2 μL of AAV-ie-GJB2 therapeutic agents were injected into the inner ears with a glass micropipette (504949, World Precision Instruments) connected to a Nanoliter Microinjection System (NANOLITER2020, World Precision Instruments). ..

    Injection:

    Article Title: Functional role of dentate gyrus calbindin-D28K in supporting neurogenesis and emotional-social behavior relevant to antidepressant action.
    Article Snippet: Under anesthesia with a mixture of medetomidine (0.3 mg/ kg, ZENOAQ, Japan, #28-0102), midazolam (4 mg/kg, Sandoz, Japan, #614243022), and butorphanol (5 mg/kg, Meiji, Japan), mouse brains were fixed using stereotaxic instruments (Narishige, Tokyo, Japan, SR-5 M-HT). .. An AAV solution of 500 nL per injection site (2.5 × 108 copies) was bilaterally injected into the DG using a Pneumatic PicoPump (World Precision Instruments, Hessen, Germany, PV820) through a glass micropipette (World Precision Instruments, 1B100–3) with a micropipette puller (Narishige, PN-30). ..

    Article Title: Combination of engineered cell type-specific promoters and a high-efficiency AAV capsid restores hearing in adult DFNB1 mice model with demonstrated safety in nonhuman primate
    Article Snippet: .. A total of 2 μL of AAV-ie-EGFP, 2 μL of AAV-ie-mNeonGreen-Bpnls or 2 μL of AAV-ie-GJB2 therapeutic agents were injected into the inner ears with a glass micropipette (504949, World Precision Instruments) connected to a Nanoliter Microinjection System (NANOLITER2020, World Precision Instruments). ..

    Article Title: Dynamic Changes in Hindlimb Motor Cortex Neurons during Simulated Weightlessness Revealed by Miniature 2-Photon Microscopy
    Article Snippet: After drilling and exposing the brain surface, the hole was immediately covered with drops of saline (NaCl 9 mg/ml, B. Braun Medical). .. At the same coordinates as the drilled hole, the adeno-associated virus was injected into the hindlimb motor cortex using a pulled glass micropipette (Cat. No. 504949, World Precision Instruments) at a rate of approximately 50 to 70 nl/min. .. The virus used was hSyn-GCaMP6s-WPRE-pA (titer, 3.41 × 10 12 vg/ml, undiluted, Addgene), with an injection volume of 0.3 μl per site.

    Article Title: Pannexin-1 channel activity regulates neurogenesis and cell survival in the developing cortex
    Article Snippet: Kits were placed on a stereotaxic frame (Stoelting, Cat. #51615) on top of a warming pad (Stoelting, Cat. #51625W) to maintain internal temperature. .. A plasmid mixture of either WT or mutant transposon (2.5μg/μl), pCAG-PBase (500ng/μl), pCAG-dsRed (1μg/μl), and 0.1% FastGreen FCF (Sigma, Cat. F7252-5G) was injected (∼3 ul) through a pulled glass micropipette (World Precision Instruments, Cat. #1B120F-4) at 2 mm below pia into the lateral (right) telencephalic ventricle. .. Following intraventricular injection, tweezers with needle and paddle electrodes (NEPA Gene, Cat. #CUY661-3X7) were used to electroporate the DNA into the ventricular zone of the developing cortex by delivering five 50-ms electric pulses of 50 V at 1-s intervals using an electroporator (BTX, Cat. #ECM 830).

    Microinjection:

    Article Title: Combination of engineered cell type-specific promoters and a high-efficiency AAV capsid restores hearing in adult DFNB1 mice model with demonstrated safety in nonhuman primate
    Article Snippet: .. A total of 2 μL of AAV-ie-EGFP, 2 μL of AAV-ie-mNeonGreen-Bpnls or 2 μL of AAV-ie-GJB2 therapeutic agents were injected into the inner ears with a glass micropipette (504949, World Precision Instruments) connected to a Nanoliter Microinjection System (NANOLITER2020, World Precision Instruments). ..

    Virus:

    Article Title: Dynamic Changes in Hindlimb Motor Cortex Neurons during Simulated Weightlessness Revealed by Miniature 2-Photon Microscopy
    Article Snippet: After drilling and exposing the brain surface, the hole was immediately covered with drops of saline (NaCl 9 mg/ml, B. Braun Medical). .. At the same coordinates as the drilled hole, the adeno-associated virus was injected into the hindlimb motor cortex using a pulled glass micropipette (Cat. No. 504949, World Precision Instruments) at a rate of approximately 50 to 70 nl/min. .. The virus used was hSyn-GCaMP6s-WPRE-pA (titer, 3.41 × 10 12 vg/ml, undiluted, Addgene), with an injection volume of 0.3 μl per site.

    Mutagenesis:

    Article Title: Pannexin-1 channel activity regulates neurogenesis and cell survival in the developing cortex
    Article Snippet: Kits were placed on a stereotaxic frame (Stoelting, Cat. #51615) on top of a warming pad (Stoelting, Cat. #51625W) to maintain internal temperature. .. A plasmid mixture of either WT or mutant transposon (2.5μg/μl), pCAG-PBase (500ng/μl), pCAG-dsRed (1μg/μl), and 0.1% FastGreen FCF (Sigma, Cat. F7252-5G) was injected (∼3 ul) through a pulled glass micropipette (World Precision Instruments, Cat. #1B120F-4) at 2 mm below pia into the lateral (right) telencephalic ventricle. .. Following intraventricular injection, tweezers with needle and paddle electrodes (NEPA Gene, Cat. #CUY661-3X7) were used to electroporate the DNA into the ventricular zone of the developing cortex by delivering five 50-ms electric pulses of 50 V at 1-s intervals using an electroporator (BTX, Cat. #ECM 830).



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